Journal: iScience

Article Title: KRT15 identified by scRNA-Seq and machine learning as stemness regulator and prognostic biomarker in ESCC

doi: 10.1016/j.isci.2026.115020

Figure Lengend Snippet: KRT15 is enriched in ESCC tumor spheroids and positively correlates with the stemness marker CD44 (A) Stem cell enrichment experiments performed in four cell lines, with images captured over time. (B and C) qPCR analysis of CD44 and KRT15 mRNA expression in tumorspheres versus adherent cells from ECA109, KYSE180, KYSE150, and TE1 cell lines. n = 3, statistical analysis performed using an unpaired t test. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (D) Western blot analysis of CD44 and KRT15 protein expression in tumor spheres compared to adherent cells in ECA109 and KYSE180 cell lines.

Article Snippet: Human: KYSE150 cells , DSMZ , ACC-375.

Techniques: Marker, Expressing, Western Blot

Journal: The FASEB Journal

Article Title: Hsa_circRNA_0101125 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma via miR ‐143‐3p/ ZNF148 Axis

doi: 10.1096/fj.202500257R

Figure Lengend Snippet: The localization of hsa_circRNA_0101125 and the expression of related genes in KYSE150 and ECA109 cells. (A) Fluorescence in situ hybridization (FISH) showing the subcellular localization of hsa_circRNA_0101125 in KYSE150 and ECA109 cells (Magnification, ×400). (B) RT‐qPCR analysis of hsa_circRNA_0101125, miR‐143‐3p, ZEB1, ZHX3, and ZNF148 expression levels in KYSE150 and ECA109 cells. * p < 0.05 compared with KYSE150. n = 3. CircRNA, circular RNA; miR, microRNA; ZEB1, zinc finger E‐box binding homeobox 1; ZNF148, zinc finger protein 148; ZHX3, zinc fingers and homeoboxes 3.

Article Snippet: Esophageal cancer cell lines KYSE‐150 (CL‐0737, Procell, Wuhan, China) and ECA‐109 (BNCC342591, BeNa Culture Collection, Beijing, China) were cultured in complete culture medium RPMI‐1640 (KGM31800S, KeyGen Biotech, China) containing 2 μg/mL puromycin (P8230, Solarbio, Beijing, China) and 10% fetal bovine serum (FBS; Gibco, New York, USA) at 37°C in a culture incubator containing 5% CO 2 and 95% air.

Techniques: Expressing, Fluorescence, In Situ Hybridization, Quantitative RT-PCR, Binding Assay, Zinc-Fingers

Journal: The FASEB Journal

Article Title: Hsa_circRNA_0101125 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma via miR ‐143‐3p/ ZNF148 Axis

doi: 10.1096/fj.202500257R

Figure Lengend Snippet: Effect of hsa_circRNA_0101125 knockdown on proliferation, migration, and epithelial marker expression in ESCC cells. (A) RT‐qPCR analysis showing the reduction of hsa_circRNA_0101125 expression in KYSE150 and ECA109 cells after transfection with sh‐hsa_circRNA_0101125. (B) RT‐qPCR analysis of miR‐143‐3p expression following hsa_circRNA_0101125 knockdown. (C) Representative images of scratch wound healing assay in ECA109 cells at 0 h and 24 h. (D‐E) Quantitative analysis of wound closure. (F) Cell viability of ECA109 cells measured by CCK‐8 assay at 24 h and 48 h after knockdown. (G) Western blot analysis of E‐cadherin protein expression in KYSE150 and ECA109 cells after knockdown. Relative expression values represent the gray intensity of E‐cadherin normalized to β‐Actin, based on densitometry analysis using ImageJ software. * p < 0.05 compared with Control, # p < 0.05 compared with NC. CircRNA, circular RNA; miR, microRNA.

Article Snippet: Esophageal cancer cell lines KYSE‐150 (CL‐0737, Procell, Wuhan, China) and ECA‐109 (BNCC342591, BeNa Culture Collection, Beijing, China) were cultured in complete culture medium RPMI‐1640 (KGM31800S, KeyGen Biotech, China) containing 2 μg/mL puromycin (P8230, Solarbio, Beijing, China) and 10% fetal bovine serum (FBS; Gibco, New York, USA) at 37°C in a culture incubator containing 5% CO 2 and 95% air.

Techniques: Knockdown, Migration, Marker, Expressing, Quantitative RT-PCR, Transfection, Wound Healing Assay, CCK-8 Assay, Western Blot, Software, Control

Journal: The FASEB Journal

Article Title: Hsa_circRNA_0101125 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma via miR ‐143‐3p/ ZNF148 Axis

doi: 10.1096/fj.202500257R

Figure Lengend Snippet: Downregulation of miR‐143‐3p reversed the effect of hsa_circRNA_0101125 on KYSE150 and ECA109 cells. (A) RT‐qPCR analysis of miR‐143‐3p expression after co‐transfection with miR‐143‐3p inhibitor and sh‐hsa_circRNA_0101125. (B) Cell viability detected by CCK‐8 assay at 24 h and 48 h after co‐transfection with miR‐143‐3p inhibitor and sh‐hsa_circRNA_0101125. (C‐D) Wound healing assay to assess cell migration after co‐transfection with miR‐143‐3p inhibitor and sh‐hsa_circRNA_0101125 (Magnification, ×400). (E) Transwell invasion assay to assess invasive ability after co‐transfection with miR‐143‐3p inhibitor and sh‐hsa_circRNA_0101125 magnification, ×400. * p < 0.05 compared with control, # p < 0.05 compared with NC, & p < 0.05 compared with shRNA. CircRNA, circular RNA; miR, microRNA.

Article Snippet: Esophageal cancer cell lines KYSE‐150 (CL‐0737, Procell, Wuhan, China) and ECA‐109 (BNCC342591, BeNa Culture Collection, Beijing, China) were cultured in complete culture medium RPMI‐1640 (KGM31800S, KeyGen Biotech, China) containing 2 μg/mL puromycin (P8230, Solarbio, Beijing, China) and 10% fetal bovine serum (FBS; Gibco, New York, USA) at 37°C in a culture incubator containing 5% CO 2 and 95% air.

Techniques: Quantitative RT-PCR, Expressing, Cotransfection, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Control, shRNA

Journal: The FASEB Journal

Article Title: Hsa_circRNA_0101125 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma via miR ‐143‐3p/ ZNF148 Axis

doi: 10.1096/fj.202500257R

Figure Lengend Snippet: Western blot analysis of EMT‐related proteins in KYSE150 and ECA109 cells following hsa_circRNA_0101125 knockdown and miR‐143‐3p inhibition. Protein levels of ZNF148, Vimentin, and E‐cadherin were evaluated by Western blotting in the control, NC, sh‐hsa_circRNA_0101125, and sh‐hsa_circRNA_0101125 + miR‐143‐3p inhibitor groups. Relative expression values represent the gray intensity of E‐cadherin normalized to β‐Actin, based on densitometry analysis using ImageJ software. * p < 0.05 compared with control; # p < 0.05 compared with NC, & p < 0.05 compared with shRNA. CircRNA, circular RNA; miR, microRNA; ZNF148, zinc finger protein.

Article Snippet: Esophageal cancer cell lines KYSE‐150 (CL‐0737, Procell, Wuhan, China) and ECA‐109 (BNCC342591, BeNa Culture Collection, Beijing, China) were cultured in complete culture medium RPMI‐1640 (KGM31800S, KeyGen Biotech, China) containing 2 μg/mL puromycin (P8230, Solarbio, Beijing, China) and 10% fetal bovine serum (FBS; Gibco, New York, USA) at 37°C in a culture incubator containing 5% CO 2 and 95% air.

Techniques: Western Blot, Knockdown, Inhibition, Control, Expressing, Software, shRNA